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. 2019 Jun 28;20:360. doi: 10.1186/s12859-019-2880-8

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© The Author(s) 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Cell segmentation efficacy and cell morphology. a histograms showing distribution of circularity and level of contact with other cells (shown as percentage of cell perimeter touching with other cells. Based on histograms, low/high circularity and isolated/growing together groups were created. b effect of cell reconstruction, on segmentation accuracy, subset of low/high circularity and low/high contact with other cells (for this step, dLoGm-Kong was used in next segmentation step for all methods). c effect of various Seed-point extraction methods, effect of low/high circularity and low/high contact on segmentation efficacy. Last step is shown for QPI data only