Fig. 1.

Tau rTg4510 mice show significant polyamine dysregulation. a Simplified polyamine pathway. Ornithine decarboxylase antizyme 1 (OAZ1), ornithine decarboxylase (ODC), spermidine synthase (SRM), spermidine synthase (SMS), spermine oxidase (SMOX), spermidine/spermine-N¹-acetyltransferase (SSAT), polyamine oxidase (PAOX), polyamine-modulated factor 1 (PMF1), polyamine modulated factor binding protein 1 (PMFBP1). b, c Representative images and quantification of western blot analysis of 12-month-old nTg and rTg4510 (n = 5) hippocampal tau neuropathology (pSer199/202: t (4⁺) = − 7.490, p = .002; pSer356: t (4⁺) = − 4.588, p = .010; and total tau (H150): t (4⁺) = − 4.201, p = .014), followed by quantification (n = 5). Independent sample t test with ⁺Levene’s test for equality of variance correction, *p < .05. Data is represented by means ± S.E.M. d–h Representative images and quantification of immunohistochemical and western blot analysis of 14-month-old nTg and rTg4510 (n = 2–4) cortical and hippocampal C-terminally truncated tau neuropathology (tau ΔD421). Data is represented by means ± S.E.M. i–j Representative images and quantification of western blot analysis of 12-month-old nTg and rTg4510 (n = 4–5) polyamine dysregulation (ODC: t (4.843⁺) = 4.320, p = .008; SRM: t (5.347⁺) = − 3.756, p = .012; SMS: t(8) = 4.412, p = .003; SSAT: t(7) = − 2.849, p = .025; SMOX: t (4.399⁺) = − 2.958, p = .037; PMF1: t (4.113⁺) = − 2.690, p = .053; PMFBP1: t(8) = 3.850, p = .005). Independent sample t test with ⁺Levene’s test for equality of variance correction, *p < .05. Data is represented by means ± S.E.M.