Fig. 2.

Co-culturing promoted the migration capacity of 786-O cells and HUVECs in an MCTs-dependent manner. a–h In the transwell culturing, 1 × 10⁴ cells were seeded in the upper chamber and 4 × 10⁴ cells were seeded in the lower chamber. The migration ability of 786-O cells (a–d) and HUVECs (e–h) was measured by evaluating the width of scratches at 24 h after culturing in the single-culturing mode and co-culturing mode. In the single culture, the cells were seeded in both the upper and lower chambers; in the HUVEC coculture, 786-O cells were added to the upper chamber while HUVECs were added to the lower chamber; in the 786-O coculture, HUVECs were added to the upper chamber while 786-O cells were added to the lower chamber; in Single + 7ACC1 or coculture + 7ACC1, 10 µM 7ACC1 was added to the culturing conditions