Fig. 4.

Co-culturing significantly increased the protein expression of MCT1 and MCT4 in both 786-O cells and HUVECs. a, c In transwell culturing, 1 × 10⁴ cells were seeded in the upper chamber and 4 × 10⁴ cells were seeded in the lower chamber. The protein levels of MCT1 and MCT4 in 786-O cells and HUVECs at 24 h after culturing were measured by western blotting. a Representative images show the protein levels of MCT1 and MCT4 in indicated cells at specified culturing conditions. b, c Summarized data shows the relative band intensity of (b) MCT1 protein (b) and (c) MCT4 protein in indicated cells in specific culturing conditions. For single culture, the cells were seeded in both the upper and lower chambers; in the HUVEC coculture, the 786-O cells were added to the upper chamber while HUVECs were added to the lower chamber; in the 786-O coculture, HUVECs were added to the upper chamber while 786-O cells were added to the lower chamber; in coculture + 7ACC1, 10 µM 7ACC1 was added to the culture medium during co-culturing. ***P < 0.001, between the indicated groups