Fig. 5.

Co-culturing significantly increased the mRNA levels of MCT1 and MCT4 in both 786-O cells and HUVECs. a, b In the transwell culturing, 1 × 10⁴ cells were seeded in the upper chamber and 4 × 10⁴ cells were seeded in the lower chamber. The mRNA levels of (a) MCT1 and (b) MCT4 in 786-O cells and HUVECs at 24 h after culturing were measured by RT-qPCR. In the control, the cells were seeded in both the upper and lower chambers; in the HUVEC coculture, 786-O cells were added to the upper chamber while HUVECs were added to the lower chamber; in the 786-O coculture, HUVECs were added to the upper chamber while 786-O cells were added to the lower chamber; in control + 7ACC1 and coculture + 7ACC1, 10 µM 7ACC1 was added to the culturing conditions. *P < 0.05, **P < 0.01, ***P < 0.001, between the indicated groups