Figure 2.

DANCR inhibition suppressed proliferation, migration, and invasion of PC cells. (A) Transfection efficiency was evaluated by qRT-PCR. (B) Cell proliferation was detected by CCK-8 assay. (C) DANCR knockdown led to cell cycle arrest at G0/G1 phase in PANC-1 and SW1990 cells. (D) DANCR knockdown promoted cell apoptosis. (E) The colony-forming ability was examined by colony formation assay. (F, G) The migration and invasion abilities were examined through Transwell assays. (H) The tumor volume was measured every week to construct tumor growth curves. (I) Tumor weight was measured when the tumors were harvested. * P<0.05, ** P<0.01, *** P<0.001.