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. Author manuscript; available in PMC: 2019 Oct 11.
Published in final edited form as: Mucosal Immunol. 2019 Apr 11;12(4):1004–1012. doi: 10.1038/s41385-019-0164-2

Figure 1. TNC fn and fbg domains contain HIV Env and variable loop 3 (V3) binding domains.

Figure 1.

A) Schematic of TNC full length and truncated recombinant proteins. TNC-L, TNC-S, TNC-Sffbg, TNC-SΔfn-fbg, fnALL+fbg, and fnALL were expressed in mammalian cells. fn1–8. fn A-D, fn 6–8, and fbg were expressed in bacteria. TNC fbg, fn1–8, fn 6–8, and fn A-D are capable of binding both B) gp120 and C) conformational V3 scaffold protein. TNC-S binding to both D) gp120 and E) conformational V3 scaffold protein is abrogated by deletion of both fbg (TNC-SΔfbg) and fbg and the fn domains (TNC-SΔfn-fbg). Either human or bovine albumin was used as a negative control binding protein in each assay. Data is displayed as the median and range of assay replicates.