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. Author manuscript; available in PMC: 2019 Nov 19.
Published in final edited form as: Mucosal Immunol. 2019 May 19;12(4):930–944. doi: 10.1038/s41385-019-0170-4

Figure 5. Alcohol administration induces pro-apoptotic signaling and ER stress in the proximal small intestine.

Figure 5.

(A) Pro-apoptotic proteins Bax, Bim, Bid, truncated Bid (tBid) and cleaved (clv) PARP, as well as ER stress related proteins CHOP, p-eIF2α, eIF2 α, GADD34 full length (fl) were measured by Western blot and quantified (B) from PSI tissue of pair-fed (PF) mice as well as mice fed a 10-day chronic alcohol, a 10d EtOH plus 4h binge or a 10d EtOH 9h binge diet. (C) Caspase-3 (fl), cleaved (clv) caspase-3, caspase-2 and caspase-8 p43 were measured from the same PSI samples by Western blot and quantified (D). (E) Mice received either calorie matched diet (PF) or 10d EtOH 9h binge and some mice from each group also received in vivo administration of the ER stress inhibitor 4-phenylbutiric acid (4-PBA). Expression in the PSI of CHOP, procaspase-8 and clv caspase-8 p43 were measured by Western blot and quantified (F). For A-D, n=5–7 mice/group; p < 0.05 * vs pair-fed (PF), # vs 10d EtOH no binge, † vs 10d EtOH 4h binge. For E-F, n=5–7 mice/group; p < 0.05 * vs pair-fed, # vs 10d EtOH no binge, † vs 10d EtOH 4h binge,0 vs 10d EtOH 9h binge.