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. Author manuscript; available in PMC: 2019 Nov 19.
Published in final edited form as: Mucosal Immunol. 2019 May 19;12(4):930–944. doi: 10.1038/s41385-019-0170-4

Figure 6. Alcohol-induced CHOP expression localizes to Paneth cells.

Figure 6.

(A) PSI epithelial and immune cell fractions (intraepithelial [IEL] and lamina propria leukocytes [LPL]) were isolated from pair-fed (PF) and 10d EtOH 9h binge mice for Western blot analysis and quantified (B) of CHOP and Bax proteins. (C) Representative CHOP immunofluorescent (IF) images of PSI samples of pair-fed (PF) and 10d EtOH 9h binge mice. Insets show individual crypts and the more intense IF staining observed in the 10-day + 9h binge alcohol samples. (E) Crypts were isolated from PF and 10d EtOH 9h binge mice and Bax and Bim proteins were measured by Western blot and quantified (F). n=3–7 mice/group; * p < 0.05 vs pair-fed. Scale bars = 50μm.