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. Author manuscript; available in PMC: 2019 Nov 19.
Published in final edited form as: Mucosal Immunol. 2019 May 19;12(4):930–944. doi: 10.1038/s41385-019-0170-4

Figure 7. Tight-junction levels in the proximal small intestine are reduced by alcohol and restored by in vivo IL-17 blockade.

Figure 7.

(A) The bacterial cell wall component LPS was measured in the livers of pair-fed (PF) and 10d EtOH 9h binge mice as well as EtOH mice treated with anti-IL-17 antibody or an isotype control.(B) ZO-1 immunofluorescent (IF) staining was completed on the PSI of these same mice and quantified. (C) Occludin was measured from the PSI by Western blot and quantified (D). mRNA expression of multiple tight junction proteins including Claudin‒1, ‒5 and ‒15, Occludin and ZO-1 were measured by qPCR from PSI. For A, n=5–8 mice/group. For B, n=6 mice/group and 5 images/mouse were quantified. For C-D, n=6 mice/group. For E, n=7–10 mice/group. * p < 0.05 vs pair-fed (PF).