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. Author manuscript; available in PMC: 2019 Jun 30.
Published in final edited form as: Ann Rheum Dis. 2017 Jan 9;76(6):1123–1132. doi: 10.1136/annrheumdis-2016-210000

Figure 5.

Figure 5

Intestinal bacterial products translocate into ankylosing spondylitis (AS) bloodstream and modulate monocyte behaviour. (A-C) Serum levels of lipopolysaccharide (LPS) (A), LPS-binding protein (BP) (B) and intestinal fatty acid-BP (iFABP) (C) are increased in the sera obtained from patients with AS compared with controls. (D-F) Percentages of CD14+ cells is reduced in peripheral blood mononuclear cells (PBMCs) from patients with AS. (D) Representative dot plot showing the percentage of CD14+ cells gated on CD45 region among PBMCs in patients with AS and controls, (E) representative histogram showing CD14 MFI in patients with AS and HCs. (F) percentages of CD14+ cells in patients with AS and controls. (G and H) Percentage of HLA-antigen D Related (DR)+ cells is reduced in PBMCs from patients with AS. (G) Representative dot plot showing the percentage of HLA-DR+ cells gated on the monocytes region among PBMCs in patients with AS and controls, (H) percentages of CD14+ cells in patients with AS and controls. (I-K) Effects of monocyte stimulation with LPS alone, sCD14 alone or sCD14+LPS on CD14+ (H) and CD14− monocytes. Combination of LPS+sCD14 increased IL-23 production only in CD14− cells (I and J). (K) Representative dot plots showing the gating strategy and the percentage of IL-23 expressing cells. Results are showed as mean (SEM).