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. 2019 Jun 29;18:116. doi: 10.1186/s12934-019-1165-2

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Growth profiles of ALE clones isolated from the end populations of the evolution experiments versus starting strains on M9 minimal medium with 20 g/L sucrose. Error bars represent standard deviation (n = 3). MG1655 construct with csc (MGcscBKA) growth profile is represented on a, MGcscBKA derivatives with cscB SNP (MGcscBKAp) mutation on b and E. coli W strain on c. Optical density measurement at OD₆₀₀ were measured on a plate reader and the common ratio between the plate reader OD₆₀₀ and a benchtop spectrophotometer with a 1 cm path length is 4.2