Table 2.

Physiological data for the evolved isolates on sucrose M9 medium and sugarcane juice M9 minimal medium (SCJ medium)

Clone/construct	Clone ID	M9 Sucrose medium	SCJ medium
		Growth rate, μ (h−1) on M9 2% sucrose	Growth rate, μ (h−1)	Final density (gCDW/L)	Sucrose uptake rate (mmol gCDW−1 h−1)	Acetate production rate (mmol gDW−1 h−1)	Biomass yield, YX/S, (gCDW g of sucrose−1)	Uptake rate fold increase vs start strain
K-12 MGcscBKA	Starting strain	0.54 ± 0.01	0.68 ± 0.03	1.47 ± 0.04	7.56 ± 0.96	4.20 ± 0.20	0.28 ± 0.01	1.00
	1	0.85 ± 0.01	0.88 ± 0.02	1.91 ± 0.03	8.71 ± 0.2	4.73 ± 0.11	0.30 ± 0.01	1.15
	2	0.63 ± 0.01	0.68 ± 0.02	1.52 ± 0.04	6.54 ± 0.23	5.67 ± 0.54	0.30 ± 0.01	0.86
	3	0.83 ± 0.01	0.86 ± 0.02	1.68 ± .0.03	7.84 ± 0.18	5.42 ± 0.13	0.32 ± 0.01	1.04
K-12 MGcscBKAp	Starting strain	0.64 ± 0.01	0.73 ± 0.01	1.48 ± 0.02	7.01 ± 1.9	4.99 ± 0.25	0.30 ± 0.01
	1	0.86 ± 0.01	0.91 ± 0.01	1.38 ± 0.02	9.89 ± 0.19	6.83 ± 0.01	0.27 ± 0.01	1.41
	2	0.88 ± 0.01	0.88 ± 0.01	1.44 ± 0.02	9.50 ± 0.01	8.27 ± 0.01	0.27 ± 0.01	1.36
	3	0.80 ± 0.01	0.82 ± 0.01	1.51 ± 0.01	8.98 ± 0.11	7.30 ± 0.09	0.27 ± 0.01	1.28
E. coli W	Starting strain	0.90 ± 0.02	0.97 ± 001	1.81 ± 0.05	9.88 ± 0.35	6.60 ± 0.49	0.29 ± .01
	1	1.10 ± 0.01	1.06 ± 0.05	1.68 ± 0.06	10.5 ± 0.47	15.90 ± 0.68	0.29 ± 0.012	1.06
	2	0.95 ± 0.01	0.96 ± 0.02	1.74 ± 0.05	14.10 ± 1.0	6.50 ± 0.51	0.20 ± 0.02	1.43
	3	0.95 ± 0.01	1.08 ± 0.02	1.92 ± 0.01	12.51 ± 1.1	7.40 ± 0.5	0.25 ± 0.03	1.27

The physiological properties of each of the clones isolated from the independent endpoint ALE experiments were compared to examine whether there were any improved phenotypic outcomes across the different experiments