Table 4.

Source of key mutations validated for causality in isolated strains from the current study or constructed previously from Lacroix et al. [22]

Genetic region	Mutation	Source
K-12 MG1655	N/A	N/A
pyrE-rph	Δ82 bp deletion	LaCroix et al. [22]
rpoB	E672K (GAA→AAA)	LaCroix et al. [22]
rpoC	R1075C (CGT→TGT)	MGcscBKAp A1 F138 I1 MGcscBKAp A2 F140 I1
	R978C (CGT→TGT)	MGcscBKAp A3 F139 I1
	T1045P (ACC→CCC)	MGcscBKA A3 F140 I1 with additional mutations of (tdcG, baeS)
pyrE-rph + rpoB	Δ82 bp + E672K (GAA→AAA)	LaCroix et al. [22]
pyrE-rph + rpoB	Δ82 bp + S621F (TCC→TTC)	MGcscBKA A1 F136 I1