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. Author manuscript; available in PMC: 2020 Jun 28.
Published in final edited form as: ACS Sens. 2019 May 23;4(6):1626–1632. doi: 10.1021/acssensors.9b00400

Figure 4.

Figure 4

Use of hsFRET to image H2S in mammalian cells. (A–C) Pseudocolor ratiometric images of HEK 293T cells treated with (A) 100 μM H2S (NaHS in DPBS, pH 7.4 as the donor), (B) 100 μM L-cysteine, or (C) 50 mg/L DL-PPG for 20 min, followed by 100 μM L-cysteine. (D–F) Quantification results for panels A–C as the mean and s.d. of all imaged cells from three independent replicates. FRET ratios were normalized to the value at t = 0 min.