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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Nanomedicine. 2019 Apr 23;19:106–114. doi: 10.1016/j.nano.2019.02.027

Figure 2. Cre-mediated recombination using PLGA NPs loaded with OH-Tam.

Figure 2.

(A) Elution of OH-Tam from PLGA(50:50) and PLGA(85:15) NPs. (B) Comparison of blood OH-Tam levels between intraperitoneal injection of free OH-Tam and intravenous injection of OH-Tam encapsulated PLGA NPs over the span of 5 days. (C) In vitro quantification of recombination within Rosa26CreER;tdTF/+ fibroblasts upon delivery of PLGA NPs containing OH-Tam. (D) Dose-dependent increase in recombination within Rosa26CreER;tdTF/+ fibroblasts (as indicated by %tdT+ cells) analyzed by flow cytometry. Numbered insets depict merged brightfield and fluorescent images of cells prior to flow cytometry analysis. (E) Comparison of Cre-recombination in Rosa26CreER;tdTF/+ mice between intraperitoneal delivery of free OH-Tam vs tail vein delivery of OH-Tam loaded PLGA NPs, bar = 100 μm. **** p < 0.0001