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. Author manuscript; available in PMC: 2019 Jun 30.
Published in final edited form as: Nature. 2018 Sep 19;561(7724):551–555. doi: 10.1038/s41586-018-0534-z

Figure 1. Defective NE assembly on lagging chromosomes.

Figure 1.

a. Non-core NE assembly defect on lagging chromosomes. Top: Experimental scheme. Bottom: Images of RPE-1 cells with lagging chromosomes (arrows, 3 experiments). Red: core NE proteins; Green: non-core proteins. b, c, Impaired nuclear import in micronuclei. b, Kymograph of RPE-1 cell (boxed region of top image) shows impaired import of RFP fused to a nuclear localization signal (RFP-NLS) in the newly formed micronuclei (arrowheads). Synchronization as in a (t=0 is anaphase onset, AO). Bottom: Merged image. Representative images of 9 cells, category “B” next panel. c, Top: Cartoon summarizing patterns of import to micronuclei (from b, see Extended Data Fig. 3a for representative quantification of import defects). Bottom: Percentage of micronuclei corresponding to the categories above (n=24, from 11 experiments for RPE-1, n=17, from 9 experiments for U2OS). Scale bars, 10 μm.