Figure 6.
Delivery of RNA fibers to human breast cancer cells triggers RNA interference. A) AFM images of different fibers functionalized at every monomer (EM) or every other monomer (EOM) with DS RNAs designed to silence GFP. B) Microscope images of human breast cancer cells expressing GFP before and after transfections with RNA fibers. C) Dose-dependent silencing efficiencies of GFP for different fibers introduced in picomolar range. D) Intracellular structural integrity of RNA fibers simultaneously labeled with two different fluorophores is confirmed by confocal microscopy colocalization experiment. E) Relative silencing efficiencies of functionalized RNA fibers (EOM) compared to functionalized RNA cubes and rings functionalized with six DS RNAs each. All assemblies in E were tested at 10 × 10−9 m concentrations as in Figure 4.