Figure 7.
RNA fibers designed to specifically downregulate expression of mutated BRAFV600E but not the wild type (WT) BRAF. A) AFM image of RNA fibers with DS RNAs at every other monomer (EOM) designed against mutant BRAFV600E and a native-PAGE showing RNA fibers assembly. B,C) Specific gene silencing triggered by RNA fibers (0.5 × 10−9 M) in human melanoma cells compared to equimolar DS RNA. Relative BRAF silencing is normalized to a house keeping gene, actin. Shown are the results for RNA fibers transfected into cell line A375 with BRAFV600E and HMCB cells with the WT BRAF gene. Error bars are ±SEM (N = 3). Statistical analysis was performed by one-way ANOVA (*p < 0.05, **p < 0.01).