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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Mol Neurobiol. 2017 Dec 27;55(8):6215–6226. doi: 10.1007/s12035-017-0836-4

Figure 2. SAP102 is phosphorylated on Ser632 by CK2 in heterologous cells, cultured cortical neurons and brain.

Figure 2

a, CK2 inhibitor TBB was applied to HEK-293 cells co-transfected with CK2 and SAP102, SAP102 ΔI2 or SAP102 S632A. SAP102 was immunoprecipitated from lysates. Immunoprecipitates were resolved by SDS-PAGE and probed with either the SAP102 Ser632 phosphorylation state-specific antibody or pan-SAP102 antibody. b, Cultured cortical neurons were treated with or without TBB for 4 hrs, and immunoblots of cell lysate were probed with either the SAP102 Ser632 phosphorylation state-specific antibody or pan-SAP102 antibody. c, P4 rat brains were solubilized, and SAP102 was immunoprecipitated from lysates. Immunoprecipitates were resolved by SDS-PAGE and immunoblotted with either the SAP102 Ser632 phosphorylation state-specific antibody or pan-SAP102 antibody. Recognition of phosphorylated SAP102 was prevented by λ-phosphatase treatment. The experiment was repeated three times and quantified using ImageQuant LAS TL software. Data represent means ± S.E. (A: *, p < 0.05, t test with Bonferroni’s correction after ANOVA; B and C: *, p < 0.05, Student’s t test).