. Author manuscript; available in PMC: 2019 Jun 30.

Published in final edited form as: J Proteome Res. 2017 Feb 14;16(3):1270–1279. doi: 10.1021/acs.jproteome.6b00871

Table 1.

Relative differences in metabolite concentrations between M. smegmatis wild-type and TAM23 alr mutant cells

No D-alanine^a,b		50 mM D-alanine
Relative Increase	Relative Decrease	Relative Increase	Relative Decrease
Alanine	α-ketoisovalerate	Citrulline	Alanine
ATP	Ethanol	Fatty acid	α-ketoglutarate
Fatty acid	GABA	Glutamate	GABA
Glucarate	Glutamate	Isoleucine	Lysine
Glycolate	Glycerol	Oxaloacetate	Ornithine
Lysine	Isoleucine	Succinate	Trehalose
Phosphoserine	Lactate	Threonine	Valine
Serine	N-acetyl-glucosamine-6-phosphate
Threonine	Oxaloacetate
	Succinate
	Valerate

Observed increase or decrease in metabolite concentrations in M. smegmatis TAM23 alr mutant cells relative to wild type mc²155 cells. Metabolites were identified from the S-plots generated from the corresponding OPLS-DA model (see Fig. 2).

Metabolites in italics exhibited a reversal in concentration depending on whether media were supplemented with or without D-alanine.