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. 2019 Jun 9;9(15):4354–4374. doi: 10.7150/thno.35125

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Effects of PEG-ss-aAPP/MTX micelles on invasion and migration of JEG3 cells. (A) Cells were scratched with a pipette tip, photographed (0 h), then treated for 24 h with 3 μM free MTX, APP/MTX micelles or PEG-ss-aAPP/MTX micelles. Control cells were left untreated. Cell migration was assessed using phase-contrast microscopy. Scale bar, 100 µm. (B) Quantitation of gap area. Ratios of the gap area relative to the gap at 0 h were plotted. (C-D) Typical images and quantitation of inhibition of JEG3 cell invasion after the treatments in panel (A). Magnification, 40×.