Figure 8.

Effects of PEG-ss-aAPP/MTX micelles on NF-κB activation in JAR and JEG3 cells. (A) Levels of IκBα and p-IκBα in total cell lysate of JAR and JEG3 cells, determined by Western blotting. (B) Levels of p65 in cytoplasmic and nuclear fractions, determined by Western blotting. Levels were quantitated relative to the levels of histone H3 and β-actin; values are mean ± SD of three independent experiments. *p < 0.05 vs. control group. (C) Localization of NF-κB immunofluorescence. JAR and JEG3 cells were treated with 3 μM PEG-ss-aAPP/MTX micelles for 6 h, and p65 was detected based on fluorescence. The experiment was performed three times with similar results, and typical results are shown. (D) Inhibition of NF-κB activity by PEG-ss-aAPP/MTX micelles based on a luciferase reporter assay. Values are mean ± SD of three independent experiments. *p < 0.05 vs TNF-α group.