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. 2019 Jun 9;9(15):4354–4374. doi: 10.7150/thno.35125

Figure 9.

Figure 9

NF-κB inhibition helps mediate the ability of PEG-ss-aAPP/MTX micelles to inhibit migration/invasion and promote apoptosis in human choriocarcinoma cells. (A) Knockdown of p65 expression by siRNA in JAR and JEG3 cells was confirmed by Western blotting. (B) Effects of p65 knockdown on the ability of PEG-ss-aAPP/MTX micelles to inhibit proliferation of JAR and JEG3 cells. Cells were transiently transfected with anti-p65 siRNA for 24 h, then treated with vehicle (0.1% DMSO) or 3 μM PEG-ss-aAPP/MTX micelles. Proliferation was determined using the MTT assay; values are mean ± SD of three independent experiments (n = 6). (C-D) Effects of p65 knockdown on the ability of PEG-ss-aAPP/MTX micelles to inhibit cell migration and invasion in cells treated as described in panel (B). Invasion was assessed using a wound healing assay, while migration was assessed using a transwell assay with or without Matrigel. (E-F) Effects of p65 knockdown on expression of MMP-2 and -9 in cells treated as described in panel (B). Transcript levels were assayed using RT-PCR; values are mean ± SD of three independent experiments. *p < 0.05, ***p < 0.001 vs the corresponding group.