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. 2019 Jun 10;9(15):4558–4566. doi: 10.7150/thno.31052

Figure 5.

Figure 5

DCRF is targeted by miR-551b-5p in an AGO2-dependent manner. (A) Bioinformatic prediction showed that DCRF sequence contained the putative binding site of miR-551b-5p. (B) The expression of miR-551b-5p was increased in HEK293 cells transfected with miR-551b-5p mimics (n = 3, *P < 0.05). (C) The DCRF cDNA was cloned downstream of the luciferase gene (Luc-DCRF-Wt) and transfected into HEK293 cells with miR-551b-5p mimics or control oligonucleotides. The miR-551b-5p binding site in DCRF was mutated to generate Luc-DCRF-Mut. Luciferase activity was detected 48 hours after transfection (n = 3, *P < 0.05). (D) The RIP assay was conducted to verify whether DCRF and miR-551b-5p directly bind to AGO2 in myocardial cells (n = 3, *P < 0.05).