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TIRF assay for the in situ analysis of miR-21 in EXs derived from different cell types. (A) TIRF images of various tumor EXs and their control EXs after introducing SDP21. Scale bar = 3 µm. (B) Number of bright spots counted in the sample region of 200 square pixels (equivalent to 53 × 53 μm). In the TIRF image, an individual bright spot was regarded as a single miR-21-EX. (C) Fluorescence intensity analysis of miR-21-EXs at the single-vesicle level in various cell-derived EX samples.
