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. 2019 Jun 9;9(15):4542–4557. doi: 10.7150/thno.35282

Figure 2.

Figure 2

Fecal microbiota transplant affects the fecal miRNA population. Three-weeks old male and female GF C57BL/6 mice were conventionalized via microbiota transplant from single WT, non-colitic IL10-/- or colitic IL10-/- female donor mice. A, Schematic representation of the experiment. B, Principal coordinate analysis (PCoA) of the weighted UniFrac distance matrix of fecal microbiota from WT (gray), non-colitic IL10-/- (orange) and colitic IL10-/- (red) microbiota-recipient mice at day 42 (D42) post-transplant. C, Taxonomic summary at the phylum level. D-E, Bioactive levels of fecal lipopolysaccharide (D) and flagellin (E) were assayed using TLR5 and TLR4 reporter cells. F, Colons of WT mice transferred with WT, non-colitic IL10-/- or colitic IL10-/- microbiota were paraffin-embedded, sectioned and stained with H&E. Bars = 100 mm. G, Histological scores. H, Fasting blood glucose. I, miRNA profiles analyzed from the feces of recipient mice at D0 and D49 post-transplant. PCoA of the expression levels of the top 12 deregulated miRNAs identified in WT (gray), non-colitic IL10-/- (orange) and colitic IL10-/- (red) microbiota-recipient mice. Data are presented as the means ± SEM (n=5 per group). Significance was determined by two-way ANOVA followed by Bonferroni post-hoc test. *, p<0.05; ***, p<0.001.