Figure 5.

Treg directly promote proliferation of mouse neonatal cardiomyocytes in a paracrine manner. Immunocytochemistry for cTnT⁺ (red) and Ki67⁺ (green), pH3⁺ (green) or Aurora B⁺ (green) cells at day 1 after coculture of (A) CD3⁺CD4⁺hCD2⁺ Treg, Treg supernatant (SN), or (G) the combination of CCL24, GAS6 and AREG (Pool 3) with mouse neonatal cardiomyocytes of P1 ICR hearts, scale bars: 50 um. Quantification of (B) the absolute number of total cTnT⁺ cardiomyocytes after cocultured for 3 days; or (C) %Ki67⁺cTnT⁺, (D) %pH3⁺cTnT⁺ or (E) %Aurora B⁺cTnT⁺ proliferating cardiomyocytes among total cTnT⁺ cardiomyocytes based on (A). Quantification of proliferating cardiomyocytes after cultured with (F) the respective paracrine factors or (H-J) Pool 3 for 1 day. Data are presented as mean±S.D., n = 3 independent experiments, *P<0.05, **P<0.01.