Skip to main content
. 2019 May 31;9(15):4265–4286. doi: 10.7150/thno.35914

Figure 7.

Figure 7

Formation of new bone-like tissue around Bio-Oss bone powder in immunocompromised mice following PDLSC implantation. (A) Masson staining of new bone and fibers. (B) Sirius red staining for identification of collagen I to IV fibers. Type I collagen shows red birefringence, type II collagen weak red light, type III collagen green birefringence, and type IV collagen weak yellow birefringence. (C, D) Immunohistochemical staining of Col-I and quantification of the Col-I-positive area. (E, F) Immunohistochemical staining of OCN and quantification of the OCN-positive area. BM, bone meal from Bio-Oss. Black bar and white bar, 200 µm. The data are presented as the means ± SD; n = 6. *** P < 0.001 represents significant differences in the indicated columns (P-dECM and B-dECM) compared with the no-ECM group. ### P < 0.001 represents significant differences between the P-dECM and B-dECM groups. +++ P < 0.001 represents significant differences between the P-dECM or B-dECM group and the P-dECM + LV-COL4A2 or B-dECM + COL4A2-siRNA group.