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. 2019 Jun 9;9(15):4461–4473. doi: 10.7150/thno.35219

Figure 4.

Figure 4

ROS-dependent inhibition of MAPK/ERK and PI3K/AKT pathways by vitamin C in thyroid cancer cells. (A) The indicated cells were treated with vehicle control or different doses of vitamin C (VC) for 2-4 h, and cell lysates were then subjected to western blot analysis to determine the effect of VC on the levels of phosphor-ERK (p-ERK), total ERK (t-ERK), phosphor-AKT (p-AKT) and total AKT (t-AKT). (B) Western blot analysis of p-ERK, t-ERK, p-AKT and t-AKT in indicated cells treated with VC or in combination with NAC with β-Actin as a loading control. A comparison of the levels of p-ERK, p-AKT and t-AKT in nude mice (C and D) and transgenic mice (E) with the indicated treatments by IHC staining. Scale bars, 100 μm. The expression levels were calculated with IOD value (lower panels), and data were presented as mean ± SD. ***, P <0.001 for comparison with the control; ###, P <0.001 for comparison with a combined treatment of VC and NAC.