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. 2019 Jun 27;7:e7167. doi: 10.7717/peerj.7167

Figure 3. Dual luciferase reporter vector construction of KLF3.

Figure 3

(A) Landing PCR products, M: D2000; the size of the amplification is consistent with the theoretical size. (B) Construction of Vector Plasmids, M: D2000; the size of the amplification is consistent with the theoretical size. (C) Vector plasmid sequencing results. (D) Vector plasmid point mutation sequencing results. (E) Dual luciferase assays with KLF3 MRE wild-type (WT) (pmiR-RB-REPORTTM -WT- KLF3) or mutant (Mut) (pmiR-RB-REPORTTM -MUT- KLF3) constructs in the presence of miR-21 mimics or NC. (F) The relative mRNA expression levels of miR-21, KLF3 between SM sheeps and STH sheeps by RT-qPCR. Data are indicated as the means ± SE derived from triplicate transfectants of three independent experiments. *p < 0.05 stands for significant difference; **p < 0.01 stands for extremely significant difference; p > 0.05 stands for no significant difference.