Table 1. Primers of targeting the seed region of the candidate target genes.
| Name | Sequence(5′–3′) |
|---|---|
| CNKSR2-W-F | GGCGGCTCGAGTACACTGCGAGAGTTGGTAGA |
| CNKSR2-W-R | AATGCGGCCGCCAAGAAGTAAGGAGAAAGTTAGG |
| CNKSR2-M-F | ACAGATTGTATTCGAAATGTTTAGAGAATT |
| CNKSR2-M-R | TAAACATTTCGAATACAATCTGTGAGTCCAATG TAG |
| KLF3-W -F | GGCGGCTCGAGAGTGTTGCACTAATGTGGA |
| KLF3-W -R | AATGCGGCCGCGAGTAGAAACAGAGAAGGAAC |
| KLF3-M -F | TATATTTTTATTCGAAATAAGACTGAATGGGTA |
| KLF3-M -R | GTCTTATTTCGAATAAAAATATATTACACTTTA |
| TNPO1-W -F | GGCGGCTCGAGTAAGATTGGATGAGTTTTATGGAG |
| TNPO1-W -R | AATGCGGCCGCACCCTCAAAACAAAAACCAAG |
| TNPO1-M -F | AATTTTTGTATTCGAGCTGAATTTTAAAGAGAG |
| TNPO1-M -R | AATTCAGCTCGAATACAAAAATTAAAACCGTAT |
Notes.
Bold letter is the mutant sequence.
- F
- forward primer
- R
- reverse primer
- W
- wild type
- M
- mutant type