TABLE 2.

Effects of any allergic sensitization (based on SPT) on DNAm via linear mixed models in the loW cohort and in the replication cohort BAMSE on each of the 35 identified CpGs

Name	Relation to CpG islands	Gene name	Location	Chr	loW cohort			BAMSE
					Est.	Raw P-value	FDR P-value	Est.	Raw P-value
cg01888561	Island	SEC14L1	TSS1500	17	−0.070	1.36 × 10−4	0.050	−0.036	0.13
cg02245534	N_Shore	METRNL	Body	17	−0.061	4.41 × 10−5	0.016	−0.060	0.14
cg02475695	S_Shore	NHLRC4	TSS1500	16	−0.077	1.22 × 10−4	0.045	−0.111	0.0026
cg02803925	—	PCYT1A	Body	3	−0.178	1.14 × 10−4	0.042	−0.136	0.016
cg03493123	N_Shore	B4GALT7	Body	5	−0.062	6.17 × 10−5	0.023	−0.012	0.69
cg05072552	N_Shore	CFL1	Body	11	−0.053	1.26 × 10−4	0.046	NA	NA
cg05390183	N_Shelf	Intergenic		1	−0.058	5.57 × 10−5	0.020	−0.053	0.010
cg06070625	—	MITF	Body; TSS200	3	−0.075	8.71 × 10−5	0.032	−0.086	0.0065
cg06099697	Island	ZFPM1	Body	16	−0.067	8.79 × 10−5	0.032	−0.024	0.44
cg07124719	—	GDEP	TSS200	4	−0.075	7.85 × 10−5	0.029	−0.064	0.031
cg07721901	—	FAM81B	TSS1500	5	−0.073	3.32 × 10−5	0.012	−0.042	0.11
cg09249800	Island	ACOT7	Body	1	−0.160	1.17 × 10−4	0.043	NA	NA
cg09705784	—	DNAH17	Body	17	−0.105	8.74 × 10−5	0.032	−0.145	0.00066
cg10159529	—	IL5RA	TSS1500	3	−0.085	5.49 × 10−5	0.020	−0.113	0.00026
cg11699125	Island	ACOT7	Body	1	−0.198	4.34 × 10−5	0.016	−0.264	0.00011
cg11988722	S_Shelf	Intergenic		20	−0.067	9.04 × 10−5	0.033	−0.086	0.0029
cg12077460	—	MFHAS1	Body	8	−0.077	2.98 × 10−5	0.011	−0.133	7.03 × 10−8
cg12105691	—	C3orf50	Body	3	−0.100	2.25 × 10−5	0.008	−0.133	8.74 × 10−5
cg14011077	—	Intergenic		9	−0.183	2.83 × 10−5	0.010	−0.235	1.16 × 10−4
cg14436861	—	WEE1	3’UTR	11	−0.089	6.74 × 10−5	0.025	−0.108	4.26 × 10−4
cg15482717	S_Shelf	FADD	3’UTR	11	−0.063	1.32 × 10−4	0.048	−0.054	0.020
cg15710961	—	DST	Body	6	−0.113	1.15 × 10−5	0.004	−0.097	0.0018
cg17203290	—	C8orf47	3’UTR	8	−0.086	4.38 × 10−5	0.016	−0.101	0.0085
cg17429587	S_Shelf	NCOR2	Body	12	−0.086	2.69 × 10−5	0.010	−0.117	1.83 × 10−4
cg17933300	S_Shelf	SCOC	TSS200	4	−0.079	1.03 × 10−5	0.037	−0.061	0.028
cg17971251	—	SEC16B	Body	1	−0.089	1.46 × 10−5	0.005	−0.159	3.38 × 10−7
cg18666454	N_Shore	KCNH2	Body	7	−0.135	1.11 × 10−4	0.040	−0.089	0.018
cg19210306	Island	C13orf27	5’UTR	13	0.217	4.63 × 10−5	0.017	0.014	0.72
cg20315954	—	PMP22	Body	17	−0.087	7.91 × 10−5	0.029	−0.097	0.0091
cg21220721	Island	ACOT7	Body	1	−0.202	1.12 × 10−4	0.041	−0.294	7.07 × 10−4
cg25087851	S_Shelf	GPR44	TSS1500	11	−0.069	1.37 × 10−4	0.050	−0.082	0.011
cg25479097	S_Shelf	C13orf35	5’UTR	13	−0.092	4.36 × 10−5	0.016	−0.093	0.0066
cg25854298	—	ASCC1	Body	10	−0.102	6.28 × 10−5	0.023	−0.131	6.38 × 10−4
cg26508444	—	FAM53B	Body	10	−0.054	1.37 × 10−4	0.050	−0.047	0.0087
cg27469152	—	EPX	3’UTR	17	−0.070	1.62 × 10−5	0.006	−0.071	0.016

Chr., chromosome; Est., estimation.

P-values that are statistically significant at the 0.05 level in the BAMSE are in bold.

The “—” in the second column represents CpGs not identified as being related to CpG islands. Following Illumina’s definition, a CpG island is defined as regions > 500 bp with more than 55% GC and expected/observed CpG ratio of more than 0.65. Around 40% of gene promoters contain islands. CpG shelves are ~4 kbp from islands, and CpG shores are ~2 kbp from islands.⁴⁷