Table 2.

List of genes and primers used for qPCR analysis.

Gene	Gene type	Primers	Tm (°C)	Amplicon size
gdh	Reference	F: 5′- TGTCGGTGAGCTGCCTAAAG −3′	60.0 °C 59.8°C	120 pb
		R: 5′- AAAGCGGTTTCAGCTAACGC −3′
pyrC	Reference	F: 5′- TCGGTAGTGAAACGGCCTTC −3′	60.0 °C 59.4°C	106 pb
		R: 5′- ATTTCTGCCGGTTTCACTGC −3′
gyrA	Reference	F: 5′- AGGTGTTCGTGGAATCCGTC −3′	60.0 °C 60.6°C	148 pb
		R: 5′- ACCGCCACGTCCTTTAACTG −3′
rpsJ	Target	F: 5′- AAGAACTGGAGCTGACGTATC −3′	57.5 °C 58.7°C	80 pb
		R: 5′- TATGAGTCGCACGAACAACTG −3′
tetM	Target	F: 5′- TGTATCACCGCTTCCGTTGG −3′	60.4 °C 60.0°C	114 pb
		R: 5′- TCGCAACCATAGCGTATCCC −3′
QP83_08660	Target	F: 5′- AAAACGGTCAGCAAAGAGGC −3′	59.3°C 60.1°C	121 pb
		R: 5′- GGTACTCCCCGTTTTCTCCG −3′
QP83_01175	Target	F: 5′- CGTTTCGACGCATGTTTTAC −3′ R: 5′- GCATTGTAGCAGTTTTAGGC −3′	56.4 °C 56.8°C	126 pb
QP83_14295	Target	F: 5′- CCGTCGGAACACTTACTGTAG-3′	56.0 °C 59.6°C	98 pb
		R: 5′- GCCAAGCTTTACTACACGTCG −3′