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. 2019 Jun 26;6(6):190266. doi: 10.1098/rsos.190266

Figure 2.

Figure 2.

Dendropanax morbiferus leaf extracts downregulated inhibitory factors and upregulated promoting factors for OL differentiation in pure OPC cultures. (a–d) OPCs were treated with EtOH- or DM leaf extract-containing medium during DIV 1–3 and were used for RNA extraction and cDNA synthesis, followed by real-time PCR to ascertain the relative expression pattern of the following genes: (a) Olig1, (b) ID2, (c) Ascl1 and (d) MBP. The expression of Olig1 and ID2 but not Ascl1 was significantly downregulated, while the expression of MBP was in a tendency to increase by DM leaf extract treatment. Bars represent mean ± s.e.m. *p < 0.05; **p < 0.01; n = 3 experiments, Student's t-test. (e) Three different cultures were pooled and used for western blot analysis to detect MBP and phospho-p42/44. β-actin was used for normalization. Compared with EtOH-treated cultures, upregulations in MBP (f) and in phosphorylation of p42/44 (g) were observed in the DM leaf extract-treated cultures.