Figure 3.

Dendropanax morbiferus leaf extract enhanced the OL differentiation in the late stage of OL development in the myelinating cultures. (a) For in vitro cocultures, OPCs were seeded on dorsal root ganglion neuronal cultures (DIV 0) and incubated with the medium containing control or DM leaf extract of the indicated ages. Fresh medium was added every two days. On DIV 7, cocultures were incubated with O4-containing medium and subsequently fixed for further staining with antibodies to MBP, Olig2 and Dapi. (b) OL developmental markers used in the analysis are indicated. Antibodies to Olig2 (blue), O4 (green) and MBP (red) stain OL-lineage cells from precursor, immature and mature stages, respectively. Image analyses were performed in the following aspects: (c) Olig2-positive cell number per field, (d) O4-positive area per an OL-lineage cell and (e) MBP-positive area per an OL-lineage cell. There were no significant changes in the early stage of the OL development by DM leaf treatment (c,d). However, DM leaf of 6- and 20-year-old but not 50-year-old significantly increased MBP-positive area per cell at DIV 7 (e). Bars represent mean ± s.e.m. *p < 0.05; n = 3 experiments, Student's t-test. Scale bar, 100 µm.