Figure 4.

Dendropanax morbiferus leaf extract increased the axonal contacts of OLs on DIV 12 in the myelinating cultures. (a–d) DRG–OPC cocultures were incubated with either EtOH (a) or DM leaf EtOH extract (b) containing medium during DIV 1–12 with regular medium changes every two days. On DIV 12, cocultures were fixed for Caspr staining to compare the axonal contacts. (c) A drawing description showing axonal Caspr clustering by OL contacts. (d) Image analysis of Caspr staining. All Caspr-positive area per cell was combined and compared between EtOH- and Dendropanax morbiferus leaf extract-treated cocultures. Bars represent mean ± s.e.m. *p < 0.05; n = 3 experiments, Student's t-test. Scale bar, 50 µm. (e) Quantitative real time PCR on the myelinating cultures treated with EtOH- or DM leaf extract during DIV 1–12. Several important myelin genes such as Myrf, CNP and PLP showed a significant increase in their expression by DM leaf extract treatment in the myelinating culture system.