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. 2019 Jul 1;152:51–57. doi: 10.1016/j.neuropharm.2018.11.009

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 2 — Quantification of GPR37-eGFP interactions with the long (D2RL-Td-Tomato) or the short (D2RS-Td-Tomato) isoform of the D2R by RCCA analysis. A) CLSM image of N2a cells expressing GPR37-eGFP (green) and D2RL-Td-Tomato (red) (left panel) and GPR37-eGFP (green) and D2RS-Td-Tomato (red) (middle panel), scale bar 10 μM. RCCA comparison between D2RL-Td-Tomato and D2RS-Td-Tomato in complex with GPR37-eGFP (right panel). B) RCCA reflecting GPR37-D2RL interactions (left panel) and GPR37-D2RS (right panel) after 48 h of treatment with 1 μM 4-PBA. C) RCCA reflecting GPR37-D2RL interactions (left panel) and GPR37-D2RS (right panel) in cells 24 h after transfection, immediately after treatment with 100 nM pramipexole. D) RCCA reflecting GPR37-D2RL interactions (left panel) and GPR37-D2RS (right panel) first treated with 1 μM 4-PBA for 48 h and then measured immediately after addition of 100 nM pramipexole. The RCCA values are means ± SD from 14 to 25 cells. *p < 0,05, **p < 0,01, ***p < 0,001 by two tailed t-test.