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. 2019 Jul 12;431(15):2894–2899. doi: 10.1016/j.jmb.2019.04.041

Fig. 1.

Fig. 1

Type III CRISPR locus of T. thermophilus HB8 (TTHB) and model structure of TTHB144 with cA4 bound. (a) Gene neighborhood of TTHB144 encoded on plasmid pTT27. Three genes encoding CARF domain-containing proteins (shown in purple) are present in the type III CRISPR locus of TTHB. TTHB152 is a Csm6 family protein, while TTHB144 and TTHB155 are hypothetical proteins of unknown function. (b) TTHB144 structure modeled using Phyre2. Each subunit of the predicted homodimer is shown by a different color (blue or cream). The highly conserved residues Thr-11, Lys-94 and His-368 are shown. (c) cA4 modeled into the CARF domain of TTHB144. Lys-94 is situated centrally beneath the cA4 molecule, and the side-chain of Thr-11 is suitably positioned to interact with cA4.