Figure 3.

FMT/CpG-pretreated MΦ supernatant reduces H1975 cell viability. (A and B) H1975 cells were incubated with different concentrations of FMT (0–400 µg/mL) or CpG (0–20 µg/mL) for 48 hrs and cell viability was evaluated with the CCK-8 assay. (C) Effect of FMT (100 µg/mL), CpG (2.5 µg/mL), FMT/CpG, the supernatant of MΦ grown for 12 hrs without stimulation (−FMT/CpG MΦS), or supernatant of MΦ pretreated with FMT/CpG for 12 hrs (+FMT/CpG MΦS) on the viability of H1975 cells after 48 hrs. Results are expressed as mean ± SDof three independent experiments. *P<0.05, **P<0.01, ***P<0.001 vs control.

Abbreviations: CCK-8, Cell Counting Kit-8; FMT, ferumoxytol; MΦ, macrophages.