Table 1.
Primer sequences used for real-time quantitative polymerase chain reaction
| Gene | Gene ID | Primer | Sequence (5′3′) | Annealing temperature (°C) | Fragment size (bp) |
|---|---|---|---|---|---|
| GAPDH | 281181 | Forward | GGGTCATCATCTCTGCACCT | 60 | 177 |
| Reverse | GGTCATAAGTCCCTCCACGA | ||||
| TLR4 | 281536 | Forward | CTTGCGTACAGGTTGTTCCTAA | 56 | 153 |
| Reverse | CTGGGAAGCTGGAGAAGTTATG | ||||
| Trx | 280950 | Forward | TGGTGTTCCTTGAAGTAGATGTGG | 60 | 109 |
| Reverse | TCACCCACCTTCTGTCCCTTT | ||||
| Trx2 | 281557 | Forward | GTTCCTGACCTCCATAATCTCCG | 61 | 121 |
| Reverse | GGCTTGGCTGGGTGTTACTGT | ||||
| Prx1 | 281997 | Forward | CGCTGGTGTCGGTCCTATTT | 60 | 98 |
| Reverse | GCTGTTGCTTTGAACTGGGG | ||||
| Grx | 515416 | Forward | TACCTCGGGTCTTCATCGGT | 55 | 103 |
| Reverse | GCTCCCATTTGCTTTAGCCG |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; TLR4, toll-like receptor 4; Trx, thioredoxin; Prx1, peroxiredoxin 1; Grx, glutaredoxin.