Figure 8. Astrocyte profiling using ex vivo model.

(A) Astrocyte extraction protocol from the cultured sections. MACS was used to extract the astrocytic cells with biotin-labelled antibody. (B) qPCR-based heat map of signature astrocytic genes that mark the different reactive state of the extracted astrocytes. Signature genes were extracted from publicly available datasets. (C) Expression analysis of reactive marker genes selected by specificity for humans and astrocytic fidelity score, in astrocytes purified from GBM patients and our human slice model with tumor injection.