Figure S4. Visualization and quantification of vasculature in glioma model.
Vibro-sections (300 μm) were prepared from the human tissue specimens, incubated for 2 wk, then postfixed and stained for collagen IV antibody. (A) Top to bottom: Shows the sections from acute, control DIV14, DIV14 GSC:CL1 and DIV14 GSC_CL2 stained for collagen IV antibody (red), tumor (pZsGreen), and DAPI (blue). Scale bars are 50 μm. (B) (i–iv) Shows the number of intersections (y-axis) and 3D distance of a blood vessel for acute, control DIV14, DIV14 GSC:CL1, and DIV14 GSC:CL2.