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. 2019 Apr 17;25:159–167. doi: 10.1016/j.molmet.2019.04.009

Table 2.

Global CpG methylome signature in relation to adipose morphology.a

Global average proportion of methylated CpG sites
 Genome distribution of differentially methylated CpG sites
No. of CpG- sites hypertrophy hyperplasia difference Pb No. of CpG sites No. of DMS % of all CpG sites % of all DMS
In relation to gene regionc
 TSS 1500 99,132 0.399 ± 0.011 0.392 ± 0.008 0.007 0.008 99,132 5591 0.173 0.159
 TSS 200 62,009 0.186 ± 0.005 0.182 ± 0.005 0.004 0.004 62,009 2015 0.108 0.057
 5′ UTR 68,096 0.449 ± 0.012 0.443 ± 0.009 0.006 0.018 68,096 4594 0.119 0.131
 1st Exon 24,866 0.217 ± 0.006 0.214 ± 0.005 0.003 0.008 24,866 833 0.043 0.024
 Body 292,753 0.662 ± 0.018 0.656 ± 0.012 0.006 0.100 292,753 20,491 0.512 0.583
 Exon Bnd 5440 0.785 ± 0.021 0.780 ± 0.014 0.005 0.241 5440 270 0.010 0.008
 3′ UTR 19,701 0.702 ± 0.019 0.694 ± 0.014 0.008 0.056 19,701 1344 0.034 0.038
In relation to CpG Islandc
 N Shelf 30,283 0.701 ± 0.019 0.696 ± 0.013 0.006 0.147 18,646 1352 0.033 0.038
 N Shore 79,745 0.446 ± 0.013 0.439 ± 0.009 0.007 0.008 64,165 4381 0.112 0.125
 CpG Island 158,706 0.184 ± 0.006 0.182 ± 0.005 0.003 0.025 133,745 2296 0.234 0.065
 S Shore 68,056 0.44 ± 0.013 0.432 ± 0.009 0.007 0.006 55,930 3901 0.098 0.111
 S Shelf 28,054 0.709 ± 0.019 0.703 ± 0.013 0.005 0.155 16,998 1232 0.030 0.035
 Open sea 455,182 0.694 ± 0.019 0.688 ± 0.014 0.006 0.103 282,513 21,976 0.494 0.625
 All 820,026 0.551 ± 0.015 0.545 ± 0.011 0.006 0.058 571,997 35,138 1 1

Values are mean ± SD.

a

Analyses of global average proportion of methylated CpG sites in relation to CpG Islands were performed in all 820,026 interrogated CpG sites. Analyses in relation to gene region and of DMS were limited to 571,997 CpG sites linked to genes.

b

Student's t test (2 sided) was used to compare global average proportion of methylated CpG sites between the two clinical groups.

c

Annotation is based on Illumina annotation of EPIC arrays; within 1500 basepairs of transcriptional start site (TSS 1500); within 200 basepairs of transcriptional start site (TSS 200); untranslated region (UTR), exon boundary (Exon Bnd); upstream of CpG island (N); downstream CpG island (S); unrelated to CpG island (Open sea).