Table 3.

Summary of selected gene displaying correlation between promoter CpG methylation, expression. and adipose morphology.^a

Gene	CpGb	Adipose morphology				Gene expression vs CpG methylatione	effect of siRNA on hMSCsf						Concordant DMS in T2D
		vs CpG methylationc		vs gene expressiond			total lipid/cell	no. lipid droplets/cell	ADIPOQ	FABP4	basal lipolysis	insulin stimulated lipogenesis
		FDR	r	FDR	r	r2
ADH1B	cg05641529	0.001	0.5	0.0001	−0.48	0.25	down	down			down	blunted
	cg24368912	0.0034	0.43			0.38							yes
	cg07059704	0.0009	0.5			0.41
AQP7	cg16447312	0.0012	0.48	0.0069	−0.34	0.18		up	down			blunted
AZGP1	cg08008696	0.0013	0.48	0.0008	−0.41	0.4	down	down	down		down	blunted
	cg26429636	0.0009	0.51			0.47
	cg13241889	0.0025	0.44			0.45
C14orf180	cg16063716	0.001	0.5	0.0022	−0.37	0.22	down	down	down	down	down	blunted	yes
	cg21198755	0.0023	0.45			0.16
	cg02868468	0.0019	0.46			0.16
	cg12981387	0.0011	0.49			0.14
	cg00497078	0.0011	0.49			0.13
	cg14803282	0.0016	0.47			0.2
	cg15665105	0.0014	0.47			0.18
	cg24102702	0.0071	0.4			0.09
	cg14735905	0.0026	0.44			0.08
	cg18484736	0.0063	0.4			0.11
	cg04220416	0.0022	0.45			0.06
CAMK1	cg01396022	0.0096	0.38	0.001	−0.40	0.07			down
	cg10836985	0.0008	0.52			0.15
	cg08237614	0.0045	0.42			0.16
GYG2	cg02075301	0.0034	0.43	0.009	−0.33	0.34	down	down			up
HADH	cg15330017	0.0024	0.44	0.0003	−0.44	0.29	down	down
	cg01573501	0.0079	0.39			0.23
PC	cg10486891	0.0044	0.42	0.0088	−0.33	0.11			down
	cg04301544	0.0093	0.38			0.1
PFKFB3	cg22224625	0.0066	0.4	0.0008	−0.41	0.15	down	down			down	blunted
	cg06002198	0.0082	0.39			0.16
	cg03889890	0.0046	0.42			0.24
	cg22692545	0.0074	0.39			0.07
	cg10405605	0.0091	0.39			0.16
PRKAR2B	cg21250978	0.0012	0.48	0.0022	−0.37	0.46	down	down			up		yes
	cg24538401	0.0018	0.46			0.43
RXRG	cg22052044	0.0063	0.4	0.0018	−0.38	0.18
	cg02364825	0.0031	0.43			0.14
	cg26419494	0.0065	0.4			0.1

^aAdipose morphology was assessed as difference between average fat cell volume and expected volume for specific total body fat. see methods for detail. For selection of genes see results section.

^bAll CpG sites listed below are located within 1500 basepairs from transcription start site.

^cRelationship between CpG-methylation, as assessed by beta-values, and adipose morphology was assessed by linear regression adjusting for age.

^dRelationship between gene expression and adipose morphology was assessed by linear regression adjusting for array batch.

^ePearson r.².

^fGenes in the leftmost column were knocked down by siRNA and effect on lipid accumulation and gene expression assessed as described in method section.