Figure 5.

Effect of skullcapflavone II on tumor necrosis factor (TNF)-α-induced MMP-1 expression in foreskin fibroblasts. Foreskin fibroblasts were incubated for 24 h in a serum-free medium with (+) or without (-) 3 μM skullcapflavone II and with (+) or without (−) 1 ng/mL TNF-α. (A) The serum-free conditioned medium and cell lysates were analyzed by 9% SDS-PAGE and Western blot with anti-MMP-1, anti-pN-ColIα1, and anti-GAPDH antibodies. (B) Cell lysates were analyzed by Western blotting using the indicated antibodies. ** p < 0.01 and *** p < 0.001 vs. the sample incubated with TNF-α alone; † p < 0.05 vs. the sample incubated without TNF-α and without skullcapflavone II; n.s., not significant.