Figure 2.

CAIX colocalizes with bicarbonate transporter NBCe1 in HT1080 cells seeded on collagen. After 5h of invadopodia assembly in 2% hypoxia, the samples were stained for NBCe1 and cortactin (A) and NBCe1 together with CAIX (B). Nuclear DAPI staining is blue. Confocal microscopy analysis revealed the occurrence of bicarbonate transporters within invading cells and their colocalization with CAIX and the invadopodial marker cortactin. The coincident distribution of both mentioned protein pairs was observed in the close proximity to the nucleus.