Table 1.
List of primary antibodies.
| Antigen | Host | Methodology–Dilution | Company |
|---|---|---|---|
| actin | g | WB - 1:1000 | Santa Cruz |
| anion exchanger 2 (AE2) | r | IF - 1:500 | GeneScript–on request [14] |
| Arp2 | r | WB - 1:1000 | Santa Cruz (sc-15389) |
| carbonic anhydrase IX (CAIX) | m | WB - 1:3 (hybridoma medium) IF - 1:250 (M75-AlexaFluor 488 conjugate) IHC - 1:100 |
in house, M75 antibody [9] |
| cortactin | m | WB - 1:1000 IF - 1:100 |
Millipore (05-180) |
| F-actin (phalloidin AF-555) | - | IF - 1:40 | Invitrogen (A34055) |
| integrin beta 3 | r | WB - 1:1000 | Abcam (ab75872) |
| Ki-67 | m | IHC - 1:100 | DAKO (M7240) |
| matrix metalloproteinase 14 (MMP14) | r | WB - 1:1000 | Millipore (AB6004) |
| electrogenic sodium bicarbonate cotransporter 1 (NBCe1) | r | IF - 1:100 | Millipore (AB3212) |
| paxillin | r | IF - 1:250 | Santa Cruz (sc-5574) |
| phospho-cortactin | r | WB - 1:1000 | Cell Signaling (4569) |
| phospho- protein-kinase A (p-PKA) | r | WB - 1:1000 | Cell Signaling (5661S) |
| protein-kinase A (PKA) | r | WB - 1:1000 | Cell Signaling (4782S) |