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qRT-PCR analysis of unigenes encoding enzymes involved in triterpenoid saponin biosynthesis. Relative expression of (A) CL2016.Contig3 (DXS), (B) CL10703.Contig.3 (DXR), (C) CL10042.Contig3 (HDS), (D) CL8457.Contig10 (PMK), (E) CL10625.Contig5 (IDI), and (F) CL7015.Contig4 (FPPS) was analyzed by qRT-PCR using the actin gene (Unigene1915) as the reference gene for normalization. Red lines indicate the FPKM values of these unigenes, and blue lines indicate the qRT-PCR results.
