FIG 7.

The region of Sam68 spanning amino acids 1 to 157 is crucial for HCV genome binding and responsible for the enhancement of viral replication. (A) The scheme of Sam68 and its truncated forms. (B, C) The plasmid encoding Flag-tagged Sam68 1 to 157 aa, 158 to 256 aa, 257 to 443 aa, or 96 to 276 aa was transfected into Huh7.5 cells for 48 h. The cells were harvested and used for co-IP with the IgG or anti-FLAG antibody. (B) RNA was extracted from the precipitate complexes and subjected to qRT-PCR. (C) Protein from the precipitate complex was detected by immunoblotting with anti-Flag antibody. (D, E) Huh7.5 cells were infected with HCV for 24 h and then transfected with Flag-tagged Sam68 truncations for 48 h followed by immunoblotting with the indicated antibodies (D). HCV RNA was determined by real-time PCR and normalized to GAPDH (E).